بررسي نشانگرهاي ژنتيكي خون در جمعيت هاي محتلف اسبان ايران : چند شكلي پروتيين آلبومين ،و...... ...

Investigation on genetic blood markers in different horse populations of Iran:Polymorphism of albumin, prealbumin, postalbumin, esterase and transferrin proteins of Arab and Caspian miniature, horses

Electrophoretic mobility of five blood plasma proteins [Albumine (Alb), Prealbumin (Pr), Postalbumin (Pas), Esterase (Es) and transferrin (Tf) were examined in two breeds or local populations of Iranian horses namely, Arab and Caspian miniature by using polyacrilamide gel electrophoresis. Gene frequencies were analyzed at five protein polymorphism loci. In addition, average heterozygosity and effective number of alleles per locus were calculated. Polymorphisms were found at five loci that brought about various phenotypes in two populations. CC and DD phenotypes in Tf system and FF phenotype in Es system were observed in Caspian miniature only. Between two populations allele frequencies difference for pr^3 and pr4 in prealbumin system was relatively high whereas, breed difference at other loci was slight. Average heterozygosity and number of effective alleles in Caspian and Arab were seen accordingly as 0.4494, 1.8654 and 0.3688, 1.5670. From the present results it can be concluded that polymorphisms of proteins which have been studied, were higher in Caspian miniature horse than in Arab breed and this has causes more heterozygosity and number of effective alleles and consequently higher genetic variabilty in this population.