ارزيابي روش DLG-ELISA جهت تشخيص سرولوژيكي توكسوپلاسموز يس تجربي در رت

Evaluation of DIG-ELISA for serodiagnosis of experimental Toxoplasmosis in Rat

In the present study, DIG-ELISA (Diffusion In Gel-ELISA) was evaluated for serodiagnosis of experimental toxoplasmosis in rat. 60 infected rat serum samples were tested using ELISA and DIG_ELiSA techniques. In DIG-ELISA, according to the diameter of reaction zone in the gei, 1:100 serum dilution was selected as a cut off. In this dilution the diameter was 3.54 mm. The results indicated that, DIG-ELISA in comparison with ELISA has high sensitivity and specificity for diagnosis of toxoplasmosis. The sensitivity and specificity of the test in 1:1 00 serum dilution, were 95% and 100% respectively. The results showed, using different concentrations of antigens for coating the plates as well as different concentrations of conjugated-antibody have not affected the diameter of reaction zone. In addition, DIG-ELISA was found fast and economical for toxoplasmosis serodiagnosis.