جداسازي و شناسايي تركيبات ارگانوفسفره و ارگانوكلره در گياه آزولاي تالاب انزلي با GC/MS

Separation and identification of organ phosphorus and organ chlorine compounds in Azolla of the Anzali Lagoon by GC/MS

In this research sampling of Azolla was done in the southwest (Siahkeshim), center (Hendekhaleh) and east (Sheijan) of the Anzali Lagoon for two seasons (Summer and Autumn 2001). Extraction of organophosphorus and organochlorine compounds in Azolla were done by mixture of methylene chloride- acetone solvents. Separation and identification of compounds in extracted samples were done by GC- NPD, ion trap and quadrupole GC/MS Sequentially. The most important pollutant identified in Azolla was Fenitrothion pesticide. Selected Ion Monitoring (SIM) technique was also used for further confirmation of Fenitrothion and the quantitative analysis was done by GC-NPD.The highest concentration of Fenitrothion in Azolla on wet weight basis was 591 ng/g in the southwest region and the lowest concentration 341 ng/g in the east region of Lagoon in Summer.