مطالعه بيوتيپ و سروتيپ PASTEURELLA MULTOCIDA جداشده از طيور ايران

Study on biotyping and serotyping of Pasteuretla multocida isolated from poultry in Iran

Biochemical patterns of twenty five P. multocida strains isolated from poultry in Iran were determined by using hexoses, pentoses, disaccarides and polyhdric alcohols. All strains were able ferment sorbitol, manitol, galactose, dextrose, fructose, glucose, Mannose, and sucrose. However each of them couldnot produce acid from dulcitol, inositol, arabinose, salicine, rhafinose and inoline. According to this pattern all of isolates belonged to subspecies (biotype) multocida. Antimicrobial sensitivity patterns were determined against 13 different therapeutic agents. Among antibiotics tested, chloramphenicol, combination of sulfamethoazine an trimethoprim and nitrofurantoion were found to be the most effective (100% sensitivity) followed by tetracycline (96%), penicilline (88%), and gentamycine (79%). Serotyping by Hedleston method showed that the isolates belonged to serotypes 1, 3, 4 and 3x4. Except serotype 1, others are reported for the first time from Iran in this research.