تشخيص ويروس بورس عفوني طيور (گامبورو) در نمونه هاي باليني و مقايسه آن با ويروسهاي واكسينال با استفاده از رويش RT-PCR-RFLP

Detection of infectious bursal disease virus in clinical samples and differentiation from vaccinal strain by RT-PCR- RFLP

In the last two decades, endemic infectious bursal disease (IBD) have been reported worldwide. 1BD is also present in Iran and heavy economic loss in poultry industry has been reported. In this study a method of RT-PCR for detection of virus in clinical samples was optimized. A 743 bp fragment of VP2 gene which is genetically variable among IBDV strains was amplified. PCR product of positive samples were digested with two restriction enzymes (RE), (Mbol and BstNI) and results were recorded. Three imported vaccine strains were also tested and results showed that RE patterns between field isolates and vaccine strains of IBDV are different. This indicates genetic variation in VP2 region of tested viruses. By using this technique, field isolates can be differentiated from vaccine strains.