روش آماده سازي سلول تخمك براي مطالعه با ميكروسكپ الكتروني

A preparation method of oocyte for electron microscopy study

Establishment of a simple, fast and reliable procedure is necessary for study of ultrastructural features of mammalian oocytes. The present study was conducted to obtain a simple and reliable procedure to examine the fine structure of mammalian oocytes and/or embryos. Excellent quality immature oocytes from apparently non-atretic camel and ewe ovarian follicles were collected and rinsed with normal saline. All steps of fixation, dehydration and embedding of the oocytes were performed using only a cavity slide. An insulin syringe and pasture pippet were used for removing and adding the solutions, respectively. Therefore, no transfer of the oocytes was required from dish to dish. The study was replicated 3 times on 8 ewe oocytes and 43 dromedary camel oocytes. The technique applied here has the advantages that makes a faster processing of the oocyte with a negligible possibility of loosing the specimen. Also obtaining high quality electron-photomicrograph of the camel oocytes was possible using this technique. Structures such as nuclei, nucleoli, mitochondria and cortical granules were preserved, photographed without any ultrastructural changes.