رابطه بين فشار اسمزي كلوييدي با غلظت پروتيين و جذب نوري ماوراءبنفش

Relationship between Colloid Osmotic Pressure,Total Protein and Colloid Absorption in Human Plasma

Background and Objectives:Determination of colloid osmotic pressure (COP) is extremely important in diagnosis and prophylaxis of several diseases. Relationship between COP and total protein (TP) concentration with constant albumin to globulin ratio(A/G) has developed previously as empirical models. The A/G variation plays an important role in COP calculation for such determination. The purpose of this study was to determine the relationship between COP,TP and colloid absorption(Abs). Materials and Methods: 300m1 human serum was obtained.Serums with different TP and A/G were prepared by adding proteins.ln order to obtain sample with different TP and constant AIG,each sample was diluted with saline.COP,TP and Abs were measured and obtained equation between these factors. For more clinical validity of such equation, serum TP of 50 hospitalized patients were measured and classified into three groups:TP<5gr/dl,5gr/dl6grldl. Abs was measured with spectrophotometer and COP measured through colloid osmometer and calculated with obtained equation. Results: The following equation was obtained as a satisfactory relationship between COP,TP and Abs in human plasma. All coefficients were statistically significant. COP = 1.8 x Abs x TP + 5.5 (r-2 = 0.92) Conclusion: Since determination of Abs is easier than measuring of A/G ratio,this proposed equation can be an easy method for calculation of COP in Emeregency rooms in which colloid osmometer not available.