اندازه گيري بعضي از آفت كشهاي آلي كلردار در آب رودخانه كارون توسط يك روش جديد از كروماتوگرافي مايع با كارايي بالا

DETERMINATION OF ORGANOCHLORINE PESTICIDES(ALDRIN, DIELDRIN DDT AND ITS DERIVATVES) IN KAROON RIVER BY HPLC

Organochlorine pesticides are important groups of chlorinated compounds, which have been used for different purposes from the beginning of the 20th century. The bioaccumulation and biomagnificantion of these compounds within various food chains is a direct consequence of their chemical stability and lipid solubility. However, even with the ban of their application by WHO and Iranian authorities, it is thought that, some of the organochlorine pesticides are still used in Iran. The aim of this investigation was to identify and determine the above mentioned organochlorine pesticides in Karoon River water by a new HPLC method. Determination of their residues in Karoon River water can be considered as an index of their presence in living organisms of Khuzestan province. In this research, samples were collected from 16 stations along Karoonr river and also from tap water in the Winter and Autumn of 1999. Extraction was carried out by liquid-liquid extraction method with n-hexane. Clean up process was done by floricil column chromatoraphy. Indentification and quantification of each component was performed by a new reverse phase HPLC method. A spherisorb (25*0.46cm) 5µm ODS column was used as stationary phase, mobile phase was composed of acetonitrile: methanol: water in ratio of 44:40:16 respectively, with a flow rate of 0.7 mL/min isocratically. A UV detector set at 218nm was used as detection system. Method validation showed that recovery, coefficient of variation, detection and quantitation limits were quite satisfactory, which are described in the paper. Results obtained indicated that the concentration of the above mentioned organochlorine pesticides in the tap water were negligible, but in the samples of Karoon river, they were in the range of 0.013 to 9.2 µg/L.