كليدواژه :
Aorta , آيورت , Vit C , ويتامين ث , Rat , موش صحرايي , فنيل افرين , كلريد پتاسيم , پزشكي
چكيده لاتين :
Background: Vitamin C is the most effective water soluble antioxidant substance. Many studies have shown an inverse relationship between the intake of vitamin C and the disorders of cardiovascular system. In this study the effect of vit C intake on contractility of isolated aorta in rats was investigated.
Materials and Methods: 60 female wistar rats wieghing 180-220g were divided into control (CG) and test groups (TG) as TG I W, TG2W, TG3W, TG4W and TG8W. They received 0.3% vitamin C in drinking water for 1, 2, 3, 4 and 8 weeks respectively. The animals were anesthetized with sodium pentobarbital (50 mg/kglip). The chests were opened and the thoracic aortas were removed. The endothelium layer was denuded and a ring of aorta (5-6 mm) was mounted in oxygenated Krebs solution containing organ bath at 37°c and pH=7.4. After a recovery period (90 min), various concentrations of KCl or phenylepherine (phe) were added. The results were reported as mean (SE g/mm2) and compared with ANOVA followed by Tukeyʹs post-Hoc test. A value of P < 0.05 was considered significant.
Results: There were no significant differences in aortic responses of CG, TG 1 W and TG2W to KCI and phenylepherine. Potassium chloride in 50, 60 and 75 mrnole concentrations in TG3W and TG4W produced tensions of 2+0.2, 2.2+0.2, 2.3+0.2, 1.96+0.2, 1.9+0.1, 1.97+0.1 g/mm2 respectively. In TG8W the same concentrations of KC1 produced tensions of 1.5+ 1,1.6+0.1, 1.6+0.1 glmm2. These tensions are significantly (P < 0.05) less than the control group. In the aorta, phenylepherine in 1, 5 micromole concentration in TG3W and TG4W produced tensions of 3.2+0.1, 3.5+0.2, 2.7+0.1, 3+0.1 g/mm2 respectively, and in TG8W produced, 2.5+0.2, 2.7+0.2 glmm2. These tensions are significantly (P < 0.05) less than control group.
Conclusion: High vitamin C intake in the diet can reduce the aorta contractility in rats.
