بررسي تجربي اثرات اسيدوز شكمبه بر الكتروليتهاي سديم ، پتاسيم ، كلسيم ، فسفر، سرم خون ، هماتوكريت خون ، نيتروژن اوره خون ، گلوكز خون ، PH شكمبه ، ميكروفلور شكمبه و ضايعات بافتي ايجاد شده در بافت شكمبه در گوسفند لري

The effects of experimentally induced lactic acidosis on serum glucose, BUN, serum electrolyte (K, Na, P, Ca), haematocrit, rumen pH, rumen microflora and pathological changes of ruminal epithelium in lori sheep

Rumen lactic acidosis in 5 clinically healthy 1-year-old male lori sheep, with 40 kg weight was successfully produce experimentally using sucrose at a dos rate of 23.5 g/kg B.W given intraruminally through a fixed rumen cannula. Three days prior to induction of the disease, rumen samples for determine normal value of microflora and rumen pH were obtained and blood collected via jugular vein to indicate baseline of haematocrit, serum electrolytes (Na, K, Ca, and 72 hours after induction of acidosis. Results indicate that heart rate (3, 6, 9, 12, 24 ,48, 72 hours) and respiratory rate increased significantly (p<0.5) at 6, 9, 12, 24 and 48 hours, body temperature decreased at 3, 6, 9, 12, 24, 48 and 72 hours. plasma sodium, plasma phosphorus and blood glucose increased at 3, 6, 9, 12 and 24 hours. plasma calcium and rumen pH at 3, 6, 9, 12 and 24 hours decreased. Blood urea nitrogen increased at 24, 48 and 72 hours, packed cell volume began to increase at 3, 6, 9, 12, 24 and 72 hours, six hours post-dosing no protozoa was found in the rumen liquor of the experimental sheep and gram positive bacteria (coccoid) were predominant at 9 hours and rod positive bacteria were predominant at 12 hours throughout the observation period. Histopathological changes in rumen of acidotic sheep indicate hydropic degeneration and erosion in ruminal epithelium.