تاثير تركيب جديدي از فنانترولين بر ميزان تستوسترون و اثرات ضد بارداري آن در موش هاي نر بالغ نژاد BALB/C

The effect of a new phenanthroline derivative on serum testosterone level and its contraceptive effects on adult Balb/C mouse strain

Background: Due to the problems arising from the overpopulation, attention has been focused on contraceptives for men. Phenanthrolines are a group of organic compounds with ionophilic characteristics and a wide range of use in biology and chemistry. In this study the effect of 2,6-diaminopyridinum was studied on hypophysis-gonad axis, testicular tissue and sperm production in male Balb/c mice. Materials and Methods: Fifty adult male BalblC mice were divided in 5 groups. First group was used as untreated control. Saline was injected to the 2nd group and the remaining 3 groups were received intraperitoneal injection of 15, 20 and 25 mg/kg of 2,6-diaminopyridinum every other day for 20 days. The LD50 was determined to be 35 mg/kg body weight. The serum concentrations of FSH, LH and testosterone were measured and the testicular tissues were studied morphologically. Results: The data showed that 25 mg/kg diaminopyridinum decreased the serum testosterone level significantly with no changes on FSH and LH levels. Histological studies on testicular tissue showed a significantly lower numbers of germ cells. Conclusion: The data obtained in this study indicate that 25 mg/kg of phenanthroline may directly affect testicular tissue causing a lower testosterone level and spermatogenesis in mice.