بررسي تنوع فتوتيپي و الكتروفورزي پكتوباكتريومهاي بيماريزا روي ذرت در استان مازندران

STUDY ON PHENOTYPIC AND ELECTROPHORETIC DIVERSITY OF PECTOBACTERIUM SPECIES INFECTING ON CORN IN MAZANDARAN

This study is aimed to evaluate the phenotypic and electerophoretic characteristics of pectobacterium strains infecting corn in Mazandaran province during 1998-1999. Infected corn samples were collected from different fields. Twenty pectobacterium strains were isolated and purified. The strains were heterogeneous based on the result of one hundred phenotypic tests. In numerical analysis of the phenotypic characteristics, two main clusters were identified. Strains of the first phenon (12 strains) with characters in common with those of Pectobacterium chrysanthemi, P.atrosepticum, P.betavascularum and P. c. subsp. atrosepticum did not match with any of the named species and subspecies. Strains of the second phenon(8 strains) were identified as P.chrysanthemi, and resembled more closely the biovar 3 of the species. The electerophoretic patterns of cell proteins of the representative strains from each phenon were cant:, fired with those of the reference strains of Pee UPB 066 and Pell SCRIl#4064.Heterogenecity in protein profiles was also noted. Therefore, atypical strains are probably members potentially new species or subspecies.