نقش ريشه در فتوسنتز، روابط آب و زنده ماني گياهان حاصل از كشت بافت آلوكك

The role of roots in acclimation and survival of tissue cultured plants of Prunus avium L.

This experiment was carried out to identify the importance of root formation in plant tissue culture technique and role of these roots in stage IV of tissue culture (Transfer to soil & acclimation). The plantlets of wild cherry, under three growth conditions (in vitro, and ex vitro - after 2 & 4 weeks) and with roots and without roots were examined for some physiological parameters. The observations showed that under in vitro conditions lack of roots had no effect on photosynthetic performance of plantlets, While, ex vitro rooted plantlets showed higher photosynthesis and Fv/Fm ratio, compared to non-rooted plantlets. The transpiration rate and hydraulic conductivity in rooted plantlets also were higher, in vitro and ex vitro, consisting with changes in stomatal conductance and photosynthesis. Under controlled conditoins of in-vitro and early stage of ex vitro growth with a high RH, a poor survival of non-rooted plantlets was observed indicating that a low photosynthetic performance may be important as loss of water.