تاثير زمان نمونه گيري ، اندازه ريز نمونه و نوع محيط بر استقرار مريسيستم هاي دو رقم تجاري انگور

The effects of culture medium, date of culture and size of explants on meristem establishment of two commercial grapevine cultivars

Meristem establishment is one of the important steps of in vitro propagation. A successful establishment of meristems, assures promising proliferation. Meristem establishment is influenced by many factors. Because of importance of Iranian cultivars and lack of enough reports on meristem culture, this study was conducted to choose the best combination of culture medium in meristem establishment of two Iranian cultivars Bidaneh Sefid and Shahroodi during 2003-2004. In the first trial, establishment of three sizes of Bidaneh Sefid cultivar meristems (<0.2, 0.3, 0.5 mm) were investigated on MS medium supplemented with 0.5 mg/I BA. In second trial, the effects of medium combination and time of culture, were investigated.The trial was done according to complete random design with three factors and three replications and the data were recorded regarding to the number of plantlets capable for proliferation, growth rate of meristems and the length of plantlets obtained from meristems after one month. The results showed significant differences between the primary size of meristems and the number of meristems and establishment capacity but, there was no differences in length of plantlets. The best results were obtained as 0.5 BA medium for Bidaneh Sefid cultivar and I.OBA+ 0.11 BA in Shahroodi cultivar both in the second half of June. So in order to have success in meristem culture, it is necessary to select proper hormonal combination to abtain the best formulation and the best time for culture.