بررسي كشت هاي درون شيشه اي گل راعي در توليد هيپريسين و ساير متابوليتهاي ثانويه

Hypericum perforatum is an improtant medicinal plant. This plant has a great potential in phramaceutical industries. In order to produce callus, seeds of "Topas" cultivar were isolated and cultured on MS medium supplemented with 2, 4-D (12.5 and 2.5mg/l) and kinetin (0.1 and lmg/1). Callus proliferation were took placed on the same medium for two months. Plantlets were regenerated in MS medium containing BAP {2.5 and 5mg/l) with NAA (2.5 and 5mg/l). The results indicated high frequency of callus induction and the plantlets regeneration of this species. Increasing of BAP concentration caused higher number of plantlets. HPLC-DAO method was used to analysis secondary metabolites of callus and in vitro plantlets. The results showed that callus with red dot on its surface, lacking hypericin and pseudohypericin, while in vitro regenerated plantlets had special nodes (black dots) containing hypericin and pseudohypericin. Production of other secondary metabolites (hyperforin, Ad-hyperforin, chlorogenic acid, Iso-chlorogenic acid and apijenin) on in vitro plantlets were higher than callus.