مطالعه تنوع ژنتيكي در جهت هاي ليراني قارچ WELTZIEN ERYSIPHE BETUE )VANHA( بيماري سفيدك سطحي چغندر با روش RDNA-RFLP

A study on genetic diversity of Iranian populations of Erysiphe betae(Vanha) Weltzien causal agent of sugar beet powdery mildew using rDNA-RFLP method

A total of 105 isolates of Erysiphe betae from different regions of Iran were investigated. For probable polymorphism of ITS-5.8S regions using PCR-based RFLP, the mentioned fragments were amplified via PCR by ITS1 and ITS4 primers and subjected to 1.5% agarose gel electrophoresis. PCR products were digested by HaeIII, AIuI, TaqI, HindIII, CfoI, EcoRI, Mspl, SacI and Rsal REs and the digestion products were resolved on 7.5% polyacrylamide. PCR product of all isolates was only one 645 bp fragment. Among nine REs tested AIuI, Cfol, Mspl and HaeIII had recognition sequences on PCR products. Because of unstability, results for AIuI were ignored.Digestion products for CfoI were 280, 185 and 180bp, for Mspl, 325, 160, 120 and 40bp and those of HaeIII, 325, 180, 75, 40 and 30bp fragments for all isolates. Electrophoretic patterns of PCR products and digested products were completely similar,thus no polymorphism was discernible between 105 isolates. Based on the results,Iranian populations of E.betae seems to be highly uniform.