بررسي تاثير تغييرات غلظت سيترات بر روي آزمايش زمان پروترومبين و وابستگي آن به زمان

Study of the Effect of Different Citrate Concentrations on Prothrombin Time and its Dependence on incubation Time

This study is designed to examine the effects of different concentrations of citrate/blood ratio on prothrombin time and its dependence on incubation time at zero and after five hours. Blood samples were randomly taken from 50 male healthy subjects and were prepared for test at citrate/blood concentrations of 0.ʹ1/1.9, (12/1.8, 0.3/1.7, 0.4/1.6. ml. At two aforementioned times, prothrombin times were determined using different concentrations and the results were compared. We found a significant relationship between different prothrombin time of different concentration ratios at zero. Their comparison five hours after incubation time did not reveal any significant correlation. The findings indicated that there were no differences between the samples as for their incubation time. Therefore, we may conclude that the time needed for sample preparation, transportation, storage and incubation at room temperature were of no significance and caused no alteration in the prothrombin time.