مطالعه آزمايشگاهي كشت سلولهاي استيوبلاست بر روي فلز تيتانيوم و مشاهده روند ايجاد رشته هاي كلاژن با استفاده از ميكروسكوپ الكترونيكي اسكينيگ SEM

Study of collagen fiber production by osteoblast cells cultured on titanium plates using SEM.

Osteoblast cells were cultured on media (N = 60) containing polished titanium plates deposited with ca2+. The plates in the control group (n = 60) were not deposited with Ca2+. Following 1, 2, 3, 5, 7 and 15 days of incubation (CO2, 37?C),the cells were detached from the plates, the plate surfaces were coated with gold and studied with SEM. Collagen fibers could be observed only on Ca2+ deposited plates from day 3 onward. The production of the fibers were time dependently increasing to day 15, hence it was not possible to detach the cells from the plates following 7 days of incubation. It is concluded that Ca2+ is essential for collagen binding to titanium surface and this may be used in surgical implantation of titanium in orthopedics,odontology and cranio-maxillo facial.