ريشه زايي ريز قلمه هاي چند رقم گردوي ايراني و تغييرات آنزيم پراكسيداز

Rooting ability of some Persian walnut cultivars and peroxidase analysis

The rooting ability of three commercial, cultivars of Juglans regia L. (ʹSunlandʹ, Chandler and Vina) was studied in vitro Microshootswere cultured on an induction medium (MS gelled with Kobe Agar and supplemented with 3 mg 1-1 IBA) for a week and rooting was evaluated after four weeks. Walnut cultivars exhibited significant differences in rooting, so that Sunland rooted most easily (90%), followed by Chandler (64%) and Vina (21%). Peroxidase enzyme has been introduced as a biochemical marker for rooting isozymes of this enzyme were analyzed in a horizontal starch gel. The presence of the peroxidase bands and polymorphic behavior was different in the easy - to - root Sunland cultivar. On the other hand, the amount of peroxidase enzyme activity was monitored during the induction stage of rooting in studied cultivars. Peroxidase activity was higher in the easiest - to - root cultivar over the rooting stages. The implication of these results in the micropropagation of walnut was discussed.