بررسي فعاليت آنزيمهاي پراكسيداز و آميلاز درختان حساس و بردار كاج در برابر خشكي در پارك چيتگر تهران

Investigation of enzymatic activity (amylase & peroxidases) of sensetive and tolerant pines against drought

Pine is one of unendemic trees in Iran. Pine is used considerably for forest plantation. For investigation on adaptation potential of pine (Pinuseldarica) against environmental factors and due to important role of peroxidases and amylase in environmental stresses, enzymatic responses of pines in forest plantation of Chitgar (Tehran) as an arid zone were studied. In some parts of Chitgar that environmental factors (for example, edaphic and climatic elements, irrigation ...) were equal, most trees have not good growth (sensitive trees) but healthy situation of some trees was suitable (Tolerant trees). Therefor after sampling from stems and laeves of both types of trees, quantitative and qualitative activities of peroxidases and amylase were analysed by spectrophotometric and electrophoresis methodes (PAGE). Results indicated that peroxidases and amylase activities of tolerant trees were lower than sensitive trees. Morever, isoenzymatic patterns of tolerant trees in comparison with sensetive trees were constant. These results indicated that, due to mismanagement in selection of seeds, and seedlings genetical differences in Chitgar pines are high.