تجزيه و تحليل استريولوژيك گلومرولهاي كليه بدنبال مسموميت مزمن با سرب در موش صحرايي آزمايشگاهي كلي يك دوره 12 هفته اي

A STEREOLOGICAL ANALYSIS OF RENAL GLOMERULI FOLLOWING CHRONIC LEAD INTOXICATION IN RATS DURING A PERIOD OF 12 WEEKS

Lead is one of the heavy metals that have an adverse effect on renal function. Its effects can involve both renal tubules as well as glomeruli. Many studies based on observation and qualitative report have been performed and some of them report changes in volume and number of glomeruli.The present study using new and unbiased stereological techniques investigated changes in glomeruli after chronic lead acetate intoxication. This study was performed at the medical school of Ahwaz medical sciences university during Autumn and Winter of 2000 and 2001. Male Wistar rats were randomly divided to four groups (n=9). During a period of 12 weeks, the treatment groups were given 0.5% and 1% lead acetate in drinking water and the control and sham control were given distilled water and 0.4% acetic acid solution respectively. Stereological analysis based on Cavalierieʹs principle was performed to determine the reference volume (VRefrence), the fraction volume of glomeruli (VvGlom) and total glomerular volume (VTGlom). Furthermore, for estimating the numerical density (NvGlom) and total number of glomeruli (NTGlom), the physical dissector was utilized. Results showed that the number of glomeruli in treatment groups had decreased significantly (P< 0.001), while for compensating this effect, the total volume of glomeruli had significant increased (P <0.001). This experiment using unbiased stereological methods confirms previous qualitative reports.