ارزيابي تاثير اوراكتومي بر روند ترميم زخم نسج نرم و سخت در رت

Evaluation of Ovariectomy Effect of Wound Healing Process in Soft and Hard Tissue in Rat

In this study wound healing process in soft and hard Tissues of the gingival area and the Mandibular bone of rats whose ovary glands were removed bilaterally (as samples which are simillar to menopause period in human) was assesed histologically and histomorphometrically and the results wire compared with those of control group. For performing this study, 20 rats were chosen and devided into two groups with ten rats in each group. In one group of rats bilateral ovariectomy was applied and in another group, simi operation was done except for ovariectomy (Sham operation). 5 months after ovariectomy, a defect was created in gingival area and Mandibular bone of lower right central incisor in 5 mm lower than gingival margin which was 5 mm deep with a 1.3 mm diameter. In 1,4,7,21 and 42 days after creation of the defect, biopsies were taken from the defect area and histologic and histomorphometric studies were done. Histological assessment sections consisted of clotting formation, degree and kind of inflammation and granulation tissue and degree and nature of bone formation. Results demonstrated that during the first days, the inflammatron was more severe in the control group rather than the study group, but gradually the inflammation process became simillar in both groups. The produced fibrous tissue in the control group was also more than the study group on the 7th day samples, Producing of new bone was obvious in the bone defect margins in both groups. On the 21st day, formation of woven bone was observed in both groups. On the 42nd day, the produced bone in both groups was lamellar, but histomorphometric studies showed that bone formation in the control group was greater than the study group. Few studies through histologic evaluation of wound healing process in soft and hard (bone) tissues after ovariectomy operation have been done. In this study wound healing process in soft tissue in ovariectomized rats has been deccelerated and the amount of new bone formation is decreased.