تشخيص ويروس تب برفكي در نمونه هاي كلينيكي به روش RT-PCR

Detection of Foot-and-Mouth disease virus in clinical samples by RT-PCR

In order to utilize a reliable, fast and sensitive test for detection of Foot-and-Mouth disease virus (FMDV) in clinical samples, a Reverse - Transcriptase Polymerase Chain Reaction (RT-PCR) method was optimized. This RT-PCR can detect FMD viral RNA in samples regardless of their serotypes. Four vaccine viral strains of types A (two isolates), 0 and Asia 1 were tested and a 131 bp pcr product from 2B region of FMDV genome was amplified. Thirty-seven clinical samples were also tested. The specificity of results was confirmed by direct sequencing of PCR products. This rapid molecular diagnostic method, which is sensitive and specific, can detect FMDV in clinical samples in less than eight hours. Therefore, it would be valuable for early detection of suspected FMDV outbreaks.