بهينه سازي رشد و توليد آلكالوييدهاي تروپاني در كشت سوسپانسيون سلولي گياه تاتوره

Growth and production optimization of tropane alkaloids in Datura stramonium cell suspension culture

Tropane alkaloids play vital roles in pharmaceutical industries . The researcher conducted the research in order to show the importance of these contents in industrial and semindustrial production of D. stramonium from solanaceae. The optimize situation for growth and production of tropane alkaloids via physicochemical factors were studied previously . Cell suspension from semiclear calli of leave explants in MS medium was obtained . MS medium contained kinetein (0.5 mg/ lit ) and NAA ( 2mg / lit ) hormones .The results obtained from the different concentrations of glucose specified that the highest level of most alkaloids production obtained by 30 g/ lit glucose . In 40 g/ lit glucose , the most biomass was produced . In different concentrations of sucrose study it was specified that the most rate of alkaloids production obtained by 20 g/ lit and the most rate of biomass production obtained by 40 g/ lit.The results showed that the concentration increased of nitrate led to the production decrease of tropane alkaloids .The best concentration of potassium nitrate for the production of tropane alkaloids was 9. 4 mM, and that of potassium nitrate for the production of biomass was 37.6 mM . Also it was evinced that the optimized concentration of ammonium nitrate for alkaloids production was 10 . 3 mM , and for the biomass was 41.22mM . The study of calcium choloride indicated that the best concentration for the growth and production of alkaloids was7.92 mM . The results obtained from the temperature study specified that the best condition for the most production of alkaloids was at 200?c and that of the most biomass production was at 250?c