كليدواژه :
Persian sturgeon , ACIPENSER PERSICUS , Formalin , Potassium permanganate , تاس ماهي ايراني , Therapeutic index , شاخص درماني , Saprolegnia , فرمالين , Malachite green , شيلات , سبز مالاشيت , پرمنگنات پتاسيم
چكيده لاتين :
Aquatic fungi (saprolegnia) are ubiquitous in natural water supplies of fish hatcheries often causing serious disease and problems. In this research, the therapeutic index of formalin, malachite green and potassium permanganate on Acipenser persicus eggs was determined and compared. Formalin, malachite green and potassium permanganate were evaluated in three trials approximately 30 hours after fertilization, using following concentration formalin-0.4, 0.8, 1.6, 3.2, 6.4 and 12.8, malachite green -0.2, 0.4, 0.8, 1.6, 3.2 and 6.4, potassium permanganate, 20, 40, 80, 160, 320 and 640 ppm. Each trial consisted of eighteen 1.8-lit aquaria for six treatments (low to high drug concentration treatment) with three replications per treatment. Therefore the total number of treatments with three untreated treatments was 57. All trials used eggs that were derived from the same egg mass with 77% fertilization rate. 100 eggs of egg mass were randomly assigned to each aquaria in order to treat eggs for 30 minutes. Then eggs transferred to separated incubator and kept there for 96 hours. During 96 hours, infected, hatched and non-hatched eggs exactly enumerated. The difference between dead eggs mean in untreated groups and other treatment used for lethal concentration calculations and the difference of infected eggs mean in untreated groups and other treatments means used lbr therapeutic concentration calculated by using pharmacologic calculation program and were determined the LC,,J, EC,o and therapeutic indices of candidate fungicides. The therapeutic indices of potassium permanganate, malachite green and formalin were 5.37, 2.25 and 0.99 respectively. In conclusion, Potassium Permanganate was shown to be safer than malachite green and formalin in controlling saprolegnia in A. persicus eggs.
