بررسي همكنش ويروسهاي موزاييك خيار و موزاييك چغندر در چغندرقند در شرايط گلخانه

Cucumber mosaic virus (CMV) and Beet mosaic virus (BtMV) cause mosaic symptoms, blistering, leaf malformation, reduction of root and foliage weight and reduction of seed yields in sugarbeet. In Iran, these viruses have been detected in single and dual infections in sligarbeet fields on different areas. In the present work interactions between CMV and BtMV, vis-a-vis symptom expression and ralative virus concentration in sugarbeet were studied. To study relative competitiveness of CMV and BtMV in mixed infections, a completely randomized design with 4 replications and eleven treatments was used under greenhouse conditions. This experiment included inoculation of sugarbeet plants with a mixture of CMV and HlMV containing different relative concentrations of the two viruses, and consecutive inoculation of plants with two viruses at 1-2 day intervals. Twenty three days after inoculation, the samples were collected and analyzed by ELISA, using respective antisera prepared in this study. Optical density(OD) values recorded by microplate reader at 410 nm were used as a measure of relative viral concentration. The data were subsequently analyzed by F test and compared with Duncanʹs test. The results showed an increase in the concentration of CMV in most doubly infected plants when compared with plants infected singly with CMV, while BtMV showed no significant change in concentration as a result of mixed infection. These results indicate a unilateral synergism between the two viruses.