رويان زايي از كشت جدايه هاي ميكروسپوري در ارقام مختلف لكزا Breassica napus l.( )

Embryogenesis from isolated microspore culture in different cultivars of rapeseed (Brassica napus L.)

Three spring cultivars of rapeseed (Global, PF and Option ) were chosen as plant materials for this experiment. Donor plants were grown in a growth chamber at a day/night temperature of 15/10°^ (16/8 hours). Microspores at late-uninucleate to early- bionucleate were isolated from buds 2.5-3.5 mm in length and cultured in modified Lichter medium (NLN-13) containing 13% sucrose. Cultures incubated at 30°^ and darkness for 14 days, then transferred to shaker in the growth chamber at 25 . Results showed that there were significant differences between cultivars at 1% level. The comparison of means showed that PF and Option, with 3412/25 and 3079/5 embryos were the best cultivars for embryogenesis respectively.