تاثير درجه حرارت بر رشد و تثبيت بيولوژيك نيتروژن گياه شبدر زيرزميني

Effect of temperature on growth and biological nitrogen fixation in subclover (Trifolium subterraneum L)

In order to study the effect of temperature on growth and biological nitrogen fixation (BNF) in subclover (Trifolium subterraneum L.) an experiment was conducted in a sand medium using hogland nutrient solution (without nitrogen) completely randomized design with 4 replication was used the experimental treatments comprised four temperatures (10, 15, 20 and 25ʹ c). This experiment was conducted at crop physiology laboratory, Gorgan University and agricultural sciences. Nodule fresh weight, nodule number, root and shoot dry weight and leaf area were measured. Results showed obtained at 10ʹc. treatment. The highest nodule number and the lowest nodule weight were observed at 15 and 25c, respectively. The highest root/ shoot ratio and nitrogen percentage in root and shoot were obtained at 15ʹc, but there was no significant difference between this treatment and 10ʹc in this respect, it was concluded that the best growth and BNF of subclover were obtained at 10-15-c.