تحقيقي در زمينه شناسايي گونه هاي TRICHODERMA در ايران

A CONTRIBUTION TO THE IDENTIFICATION OF TRICHODERMA SPECIES IN IRAN

The aim of the present study was to identify Trichoderma species isolated from Iran. Soil samples were collected from different parts of Iran focusing on the agricultural fields. Trichoderma selective media and malt extract agar (MEA) were used to isolate Trichoderma species from the soil samples. Petri plates were incubated at 25°C in the dark for the first 24 hours and then 12/12 (dark/flourescent light) at the same temperature. All the cultures were purified on 2% water agar by hyphal tip method prior to morphological examination. Morphological observations were carried out on the cultures grown on 2% MEA at 20°C under ambient laboratory conditions. Microscopic features of conidiophores and shape and size of conidia were studied and recorded 3-5 days after inoculation. Out of 350 obtained isolates, using morphological features ten species (T. citrinoviride Fig. 1, T. longibrachiatum, Fig. 2, T. saturnisporum Fig. 3, T. hamatum, Fig. 4, T. harzianum, Fig. 5, T. inhamatum, Fig. 6, T. tomentosum, Fig. 7. T. virens, Fig. 8, T. asperelluin, Fig. 9 & T. koningii, Fig. 10) were identified. Among the species T. harzianum with 150 and T. virens with 57 isolates were the most frequent species.