دروگ گيري درون گونه اي در صنوبر پده EUPHRATICA OLIV POPULUS با استفاده از روش درون شيشه اي نجات رويان

Intraspecific hybridizationin Populus euphratica Oliv. Using in vitro embryo rescue technique

In order to investigate the effect of different geographical origin of pollen grain in intraspecific hybridization and to achieve hybrids vigor, artificial hybridization has been carried out between different local genotype of Populus euphratica Oliv. from some wild growing stands of Iran. Because of long period required for embryo development on maternal plant, 45 days old ovary and ovule were used for in vitro culture. The isolated ovary and ovule were then transferred to the different kind of media (MS, Half MS and DKW ). Significant difference (p=0.05) were observed between efficiency of pollinated ovaries (34.03%) and ovalses (47.47%) to produce plantlets. In spite of no significant difference between media used for embryo germination , the highest percentage of embryo germination was observed in MS medium (43.22%). Due to existing several number of ovales in each ovary (15-30), the highest number of plantlets was observed in ovary culture. No significant differences were observed between different origin of pollen grain (geographical regions ) on precentage of embryo fertilization and germination. One hundred thirty seven plantlets were successfuly acclimatized in a green house and transfered to the Field.