بررسي حضور ژن هاي ENBP و FG BP در ميكروارگانيسم هاي موجود در كانال ريشه دندان هاي عفوني به روش PCR در بيماران مراجعه كننده به بخش اندودانتيكس دانشكده و دندانپزشكي دانشگاه علوم پزشكي شهيد بهشتي در سال تحصيلي 81-80

Investigating the presence of FnBP & FgBP Coding genes with PCR method ink microorganisms of infected root canal in patients referred to Shahid Beheshti Endodontic Department in 2000-2001

Purpose: Infective Endocarditis (IE) is a microbial infection of the endocardial surfaces usually involving the cardiac valves. Most cases of IE are caused by streptococci which from a larg part of the normal oral flora. Staphylococci are the next most cause. These organisms are well equipped with surface determinants such as Fibronectin-Binding Protein and Fibrinogen-Binding Protein which mediate their adherence to the cardiac vegetations. In this study, the presence of these two cell-surface proteins have been detected by the polymerase chain reaction technique (PCR). Method & Materials: This research is discriptive type and all the samples were selected from the adult patients referred to shahid Beheshti Dental School. Fifteen people were selected with no systemic disease or any antibiotic consumption during the last two weeks of sampling. Regarding the teeth studied, none of them had sinus tract, pulp exposure, periodontal disease or deeply broken restorations with wide open margins. Results: Results show that, of 15 root canal samples, 11(73.3%) were positive for FnBP and 8(53.3%) were positive for FgBP. Regarding the endodontic treatment adjuncts like local anaesthetic administration, rubber dam placement or saliva control, we have also taken samples from gingival sulcus, caries and saliva in 11 patients of those 15 people. The relative frequency of FnBP in gingival sulcus, caries and saliva was 72.7%, 45.5% and 54.5% respectively and for the presence of FgBP in the same samples was 63.6%, 54.5% and 36.4% respectively. Conclusion: IE prevention in succeptible patients is a duty, but it is better for dentists to use preventive cares in all jmnacted natients.