بررسي اكولوژي فلبوتومينه ها در يك كانون اندميك ليشمانيوز جلدي در شهرستان اصفهان

ECOLOGY OF SANDFLIES IN THE FOCUS OF JARGHOOYEH ZOONOTIC CUTANEOUS LEISHMANIASIS , ISFAHAN PROVINCE (SEASONAL ACTIVITY, NATURAL INFECTION, SUSCEPTIBILITY TEST)

Following an epidemic of zoonotic cutaneous leishmaniasis (ZCL) in the Jarghooyeh rural district, southeast of Isfahan, an entomological survey was conducted during 1996-98. Sandflics were collected biweekly from human dwellings and rodent burrows using sticky (oiled) paper traps. Some blood-fed and gravid female sandflies were examined for the presence of leptomonad. The susceptibility status of Phlehotomus papatasi to DDT 4% was studied in the field surveys following the WHO standard method. Impregnated papers were supplied by WHO. A total of 6266 sandflies were collected and six species were identified. The predominant species were ?papa/as/ indoors and P.ansarii in rodent burrows in the area. Natural leptomonad infection was found in P.papatasi (4.8%), P. caucasicus (12.5%), P. ansarii (7.7%) and Sergentomyia sintonl (2%) from rodent burrows, and only in P.papatasi (3.7%) from indoors. Leishmania major zymodeme MON-26 was isolated from naturally infected P.caucasicus in the rodent burrows. Susceptibility tests carried out with 4% DDT showed that P.papatasi is susceptible to this insecticide. This species was found to be resistant to DDT 4% in Bihar (India) and Turkey, and also tolerant to this insecticide in Borkhar rural district. Isfahan province.