بررسي فعاليت فاكتورهاي ويرولانس در هليكوباكترپيلوري

Study of the Cytotoxie Effecfs of the Bast Virulence Factor in Helicobaoter Piylori

Helicobacter pylori , found in the mucus of epithelial cells of the stomach , can cause mucous changes , cell lysis and inflammation . It contains such virulent factors as spiral form , motility , Hp adhesive molecules , urease enzyme (UE) and a few others . UE is of paramount importance due to its hyperactivity . This study will focus on the role of UE in causing cell lysis in different samples . 80 positive samples , out of 100 samples , were examined and all were observed to contain oxidase , catalase and UE . Curved spiral shapes were observed in direct and pathological slides which were studied . The direct effect of HP Suspension on cell culture was observed in 50 samples by adding 20 m.molar of urea . Adding bacterium suspension to 20 m.molar of urea caused the color of the culture to change into dark purple , which indicated the production of a considerable amount of ammonia (HH3) . Also , intercell vacuol (ICV) formation was observed . However , no such formation were observed without urea . The effects of different concentrations of ammonia (7 , 21 , 32 and 40 m.malar) on the formation of ICV were studied and the results indicated that ICV was not observed with NHc concentrations of less than 7 m.molar . In densities of 8 m.malar NH3 , cytopathic effects were gradually observed in the form of vacuols and increase in the number of ICV . In 21 m.malar NH3 , a few cells were damaged ; however , in densities of 32 and 40 m.molar NH3 , complete cell lysis appeared .