بررسي سرمي آلودگي با هرپس ويروس 1 و 4 اسبي در استان چهارمحال و بختياري به روش الايزا

A serological survey on equine herpes virus 1 and equine herpes virus 4 in the horse using ELISA.

This study was carried out to determine the rate of infection of horse with equine Herpes virus 1 and equine herpes virus 4 in during a 6 months from summer to the winter of the 2002. 174 serum samples were taken from different horses in Chaharmahal & Bakhtiary province of Iran. All of the samples were tested by indirect ELISA and we have determine 68 positive samples with EHV-1 (%39.08) and 120 positive samples due to EHV-4 (%68.96). The rate of infection with EHV-1 in summer %41.17 and in autumn %33.33 were determined. In summer season the infection rate with EHV-4 was %59.80 and in autumn was %81.94. In male horses the rate of infection with EHV-1 was % 15.38 ,while the infection rate with EHV-4 was %30.76.In female horses the infection rate with EHV-1 was %40.99 and the rate of infection with EHV-4 was %72.04. The rate of infection of horses which they had respiratory problems in the past was %41.79 with EHV-1 and in the horses that did not have any record of respiratory disorders was %37.38.The infection rate with EHV-4 for the horses which had the recorol of respiratory problems was %76.11 and it was %64.48 for the horses that did not have any respiratory disorders in the past. The horses less than one year old had %27.50 of infection with EHV-1. The horses that were five years old or more,the rate of infection with EHV-1 was %62.50. The infection rate with EHV-4 for the horses less than one year old was %62.50 , but the horses which were four years old,the rate of infection with EHV-4 was %74.28. When Chi - square test and Fisherʹs exact test were done,factors like,season,sex and record of respiratory problems,caused a significant difference in the rate of infection with EHV-1, but these factors did not have any effect on the rate of infection with EHV4.