بررسي اثر هم كشتي سلول هاي ك ومولوس در مايع فوليكولي بر جنين هاي يك سلولي موش

Follicular fluid and cumulus cells synergistically improve mouse early embryo development in vitro

Background and purpose: Despite the rapid development of assisted reproduction techniques (ART) in recent years, implantation rates after replacement of embryos into the uterine cavity remains low. An important approach to improve embryo quaility and implantation rates has been the use of coculture systems. Hence this study was undertaken to evaluate the influence of cumulus cells monolayer in follicular fluid on mouse early embryo development in vitro. One cell embryos were obtained from Swiss white mouse after superovulation with HMG and hCG. Cumulus cells were prepared from mouse egg-cumulus mass after a short exposure to medium containing hyaluronidase. These cells were seprated from red blood cells using a percoll gradient. Follicular fluid was collected from patients undergoing an IVF program during oocyte pick-up. Granulosa cells monolayer were prepared in Follicular fluid (FC) and Hamʹs F10 medum (HC). Mouse one-cell embryos were cultured in FC, HC, Hamʹs F10 (HF) and follicular fluid (FF) for 120 hours. Only 10% of embryos passed the 2-cell growth block in HF. Howerer, the proportion of 2-cell block passed emboryos were 21%, 27% and 69% in FF, HC and FC respectively which are significantly different from HF (P<0.05). The differences between FC and two other treatment were also significant (P<0.00 I). In FC, 35% of 4-cell embryos continued to grow to blastocyst stage whereas only 8.9% and 7.7 % of 4-cell embryos in HC and FF reached this stage and no embryos developed to blastocyst in HF. The blastocyst rate in FC was significantly higher (P<0.001). It can be concluded that follicular fluid and cumulus cells in monolayer form may synergistally improved early embryo culture conditions compared with all other treatments.