مطالعه ساختار ژنتيك جمعيت ماهي صبور در آب‌هاي خوزستان با استفاده از روش مولكولي RAPD

A Study on population genetic of Hilsa shad, Tenualosa ilisha, in Khoozestan, Iran using molecular method (RAPD)

The genetic structure of Hilsa Shad Tenualosa ilisha in Khouzestan waters including Karoon, Arvandrood and Bahmanshir Rivers as well as Persian Gulf was studied usingRAPD technique. After optimizing PCR condition, nine RAPD primers were selected from which 58 polymorphic loci were obtained on 12 specimens from each geographical region (A total of 48 specimens). RAPDPLOT, RAPDDIST and POPGENE computer software were used to analyze the RAPD data. Canonical discriminant analysis was deployed for statistical assessment of the RAPD data. Maximum and minimum genetic distances were found between samples from Arvandrood River and Persian Gulf (0.1987) and Arvandrood and Bahmanshir Rivers (0.0852), respectively. The UPGMA dendrogram showed that the samples from Karoon River and Persian Gulf form one group and samples from Arvandrood and Bahmanshir Rivers form another suggesting the hypothesis that there are Iranian and Iraqi populations of the species that chose their own specific rivers for spawning. According to this hypothesis, the specimens from Persian Gulf ehose Karoon as their spawning river. Other populations migrate to Tigris and Euphrates Rivers in Iraq. The canonical discriminant analysis of the RAPD data indicates that samples from the four geographical regions are statistically different from each other and high correlation was found for within region data (P