اثر گنادوتروپين جفتي انسان برميزان تخمك‌هاي بالغ شده انسان در محيط كشت و جنين‌هاي هشت سلولي ايجاد شده به روش تزريق اسپرم به سيتوپالسم تخمك‌

The Effect of HCG on in Vitro Maturation of Human Immature Oocytes and Rate of 8 Cell Embryos Mediated by ICSI

Introduction & Objective: The infertility treatment by USing in vitro matured oocyte has many potential applications. It minimizes the nsk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for IVF. This method may prove important for subjects in need of fertility preservation and also provides information about the final stage of oocyte maturation. The aim of this study was to determine the effect of HCG on the rate of in vitro matured oocyte and 8 cell embryos mediated by ICSI. Materials & Methods: This experimental study was done in Montaseneh IVF center and Islamic Azad University at Mashhad. One hundred sixty eight Immature oocytes aspirated from women undergoing IVF cycles were divided into 3 equal groups and allocated for maturation in 3 medium cultures. All of 3 culture groups contained G1 medium supplemented with HSA 10% (Human Serum Albumin). Control group had no gonadotropin. but the first experimental group contained HCG 10 IUiml and the second experimental group contained HCG 5 IU/ml. Matured oocytes were fertilized by ICSI and numbers of 8-cell embryos were investigated 72 hours after fertilizatron by invert microscope. Results: The results showed that both of the HCG concentrations significantly Increased the rate of matured oocytes and also the rate of 8cell embryos with respect to control group (p<0.05). No significant differences were found in rate of matured oocytes between the two experimental groups (p.-O 05) whde the number of a-eel! embryos was significantly higher in HCG 51U/ml with respect to HCG 10lU/mi and control group (p