بررسي سميت سرب بر سلول‌هاي استيوبلاستوماي انسان )SAOS-2( در محيط كشت‌

Evaluation of lead toxicity on human osteosarcoma cell line Saos-2

Lead poisoning is a problem of developing countries. The most accumulation of lead has been reported in the bone with a half-life around 20 years. Althouph the lead toxicity is widely investigated in animals, a few studies reported the toxicity of this metal in the osteoblast cell culture. In this study, the effects of short and long term lead exposure on the growth of Saos-2 cells were studied by XTT assay. Long term exposure was performed by three pulses at the 48h intervals for six days. Furthermore, effects of fetal bovine serum (FBS) on the toxicity of lead were evaluated by the above method. Results demonstrated a significant inhibition of growth in the presence of 10 and 100 ju lead acetate. The lead toxicity increased with decrease of FBS. Lead (1 fi M) induced a considerable decrease of growth in the culture medium containing 2.5% FBS. Moreover, repeated exposure to 1, 0.1 and 0.01 ju M lead resulted in 70, 42, and 22% decrease of growth, respectively. In this study, 10//M lead acetate alters the cell shape without consideration of FBS level from flat and adherent to spherical, and this alteration was seen at the lower concentrations of lead with the decrease of FBS level. In addition, lead exhibited strong toxic effects on the osteoblastoma cells in the culture medium. The results of the present study are in accordance with the studies which reported decrease in the bone density in the lead-treated animals, and focus on the direct effect of this metal on the cellular and molecular structure of the bone tissue.