كلنيزاسيون اپي فيت و آندوفيت PSEUDOMONAS SYRINGAE در گوجه فرنگي

Epiphytic and endophytic colonization of Pseudomonas syringae in tomato

Epiphytic and endophytic colonization of some Pseudomonas syringae pathovars in leaves, stems and roots of tomato (Lvycopersicon esculentum cv. Cannery Row) was studied. Surface disinfestation with chloroform revealed that the chemical is a potent surface sterilant but would not penetrate into the natural openings present on plant tissue surfaces. Hence chloroform appeared to be harmless lo massive bacterial populations present in such area as stomata and Ienticels. This chemical was found suitable for surface disinfestation in studies dealt with endophytic colonization. Plastnid pLA LacZ was introduced via triparental pairing into wild type and hrp mutant strains of p.s pv syringae, p.s. pv tomato and p.s. pv phaseolicola. Tamato cv. Cannery Row seedling were inoculated with the wild type and mutant strains of P.syringea. Microscopic examination thin sections of the inoculated but symptomless tomato organs and tissues revealed that bacterial colonization occurred mostly endophytically. P.syringae cells were obseved among paranchyma cells and in vascular tissues of inoculated plants. These sites appeared to be more suitable for colonization P.syringae.