توليد اسپاون هيبريد در قارچ خوراكي تكمه اي سفيد AGARICUS BISPORUS به منظور افزايش عملكرد

Producing high yielding hybrid spawns for Agaricus bisporus

In spite of highly economic importance of white mushroom (Agaricus bi.ytoru,c) and its world wide production, the breeding difficulties for yield improvement of this edible mushroom which is due to its biology is still under consideration. In this research the was of improvement of the mushroom fir yield by employing hetrosis phenomenon of interbreeding crosses was investigated Spore prints of six commercials exotic and two cultivated domestic strains were prepared and after 15 to 25 days, LUU different spawn produced_ In the above stages basidiospore variation in terms of -growth rate and type of colony on PDA medium and wheat spawn was carefully investigated. Fruiting test was conducted to isolate homokaryons from hetrokaryons. One hundred Isolates were classified into four groups of high, medium, low and zero in terms of yield. The relationship of these yield classes with the type of colony was studied. Those isolates with zero yields were used in interbreeding crosses to produce hybrids. Some hybrids performed significantly better both in PDA culture medium and in the wheat spawn.RAPD markers used to briefly investigation of variation between some isolates of A i 5, showed high and positive correlation between number of bands and yield for each isolate.