مرکز منطقه ای اطلاع رساني علوم و فناوري - تهيه نيوزومهاي حاوي كلرامفنيكل سديم سوكسينات و بررسي خصوصيات فيزيكوشيميايي و ضدميكروبي آنها

چكيده لاتين :

Objectives: Various systems such as microparticulate systems are utilized for intracellular chemotherapy. One type of these systems is niosomes which are categorized as vesicular systems and essentially composed of non-ionic surfactants and cholesterol. Methods: In this study non-ionic surfactant vesicles (niosomes) from three polyoxyethylene alkyl ethers, i.e. Ci6EO2 (Brij 52), C18EO2 (Brij 72), C9=9EO2 (Brij 92) and cholesterol encapsulating chloramphenicol sodium succinate (CMP) were prepared by film hydration method. In vitro characterization of niosomes including microscopical observation, size distribution measurement by laser light scattering method, release of CMP in phosphate buffered saline (PBS), pH 7.4 and Minimum Inhibitory Concentration (MIC) determination of free and entrapped antibiotic against E.coli were evaluated. Chloramphenicol concentration was determined by UV spectrophotometry at 276 nm. Results: Log-normal size distribution was observed for all prepared niosome formulations. In the presence of 40 molar percent of cholesterol the release of CMP was best fitted by Baker & Lonsdale model indicating a diffusion based release of antibiotic. In formulation containing 30 molar percent of cholesterol the erosion and dissolution based model (Hixson-Crowell) was best applicable. MIC of encapsulated CMP was less in vesicular systems in comparison with free drug. Morphological study of vesicles revealed different shape and size niosomes which were more as MLVs (Multi Lamellar Vesicles). Brij 92 containing niosomes had less stability, possibly due to liquid nature of this surfactant. Conclusion: Niosomes can be used for controlled release of chloramphenicol. However, although MIC of entrapped antibiotic is less than free one, more studies on in vivo and cell cultures such as mouse macrophages (J774) will be required in future studies.