اثرات حفاظتي فلاونوييدها در مقابل هموليز گلبولي ناشي از راديكال هاي آزاد

Protective Effect of Flavonoids, Against Red Blood Cell Hemolysis.

Flavonoids which are polyphenolic substances are found in different vegetable and fruits and they have anti-oxidant properties. Epidemiological studies showed that flavonoids reduce the prevalence of cardiovascular disease. On the other hand, the usage of synthetic antioxidants have been limited because of their toxicity and, there are different researches to find better natural antioxidants. This survey investigates the effect of some pure flavonoids such as kaempferol, quercetin, morin and rutin on red blood cell (RBC) hemolysis and their -SH capacity as membrane protection indicator. The flavonoids solutions were prepared. Rate of RBC hemolysis and -SH capacity of cell membrane were determined by spectrophotometer. 2,2ʹazobis2 amidino propane dihydrechloride (AAPH) was used to induce RBC peroxidation. The effect of each flavonoids on RBC hemolysis was examined in 3 concentrations (0.15, 5, 10) jug/ml but for investigating the effect on -SH groups only the highest concentration (10 fig/ml) of each flavonoids were used. In all cases, the antioxidant activity was dose-dependent. Rutin showed the highest inhibitory effect on RBC hemolysis than other flavonoid, that was 42.5. Also kaempferol, rutin, morin protect -SH groups 6%, 23.3%, 26.4% respectively. Results showed that flavonoids and plants contain flavonoids can be used as natural antioxidants for treatment and prevention of diseases which their pathogenesis are lipid peroxidation.