تركيب اسانس و آثار آنتي باكتريال عصاره هيدروالكلي و اسانس گياه آويشن باريك )ZIZIPHORA CLINOPODIOIDES: LAM( بر باكتري هاي منتخب

Essential Oil Composition and Antibacterial Effects of Ziziphora clinopodioides (LAM) on Selected Bacteria.

Introduction: The plant of Ziziphora clinopodioides (LAM), which is habitant of Iran, has been used in Iranian Traditional Medicine for treatment of some infectious conditions. In this survey its antibacterial effects were examined against some bacterial species. Methods and Materials: Hydro-alcoholic extract of plant obtained by solvent (methanol 70%) and essential oil obtained by hydro-distillation procedure. The extracted materials were tested against S. aurous (ATCC25923), S.pyogenes (PTCC1470), E. coli (PTCC1269), S.typhimurium (PTCC 1609), Klebsiella pneumonia (PTCC 1053), and P.aeruginosa (PTCC1430). Standard agar diffusion method was used for antimicrobial assay of methanol extract and essential oil. MIC and MBC were determined by using macro broth dilution method. Compounds of essential oil separated and identified by GC and GC/MS analysis. Results: Tow Gram positive organisms were inhibited by methanol extract at a concentration of 25mg/well, but it dose not have any inhibitory effect on 4 tested Gram negative species. Essential oil halted the growth of all tested Gram+ and Gram negative organisms, with highest effect on S.typhimurium (with MIC and MBC of 225jLig/ml). Gas chromatographic analysis revealed 22 different compounds in essential oil which five of them comprise more than 73% of oil and pulegon is the highest. Conclusion: The results suggest that Ziziphora clinopodioides has potent antibacterial effects on some Gram positive and Gram negative bacterial species especially on Salmonella typhymurium.