مقايسه اثر سميت سلولي MTA, PORTLAND CEMENT ، ROOT- MTA بر روي سلولهاي منونوكلير خون محيطي انسان

Comparison of the cytotoxic Effect of MTA, Root - MTA and Portland Cement on human peripheral blood mononuclear cells

Background & Aim: Mineral Trioxide Aggregate (MTA) is being widely used for root - end filling, pulp capping, perforation repairs, and other endodontic procedures. MTA, Portland cement (PC) and Root - MTA have many similar physical, chemical and biologic properties. PC has shown promising potential as an endodontic material in several in vitro and In vivo studies. The purpose of this study was to compare the cytotoxic effect of MTA, PC and Root - MTA on human peripheral blood mononuclear cells. Methods & Materials: In this experimental study three materials were inserted inside culture plates which contained human peripheral blood mononuclear cells. After 24 hr, the cell morphology and cell counts were studied. The viability of cells was also evaluated in 24 - hr. The results were analyzed by ANOVA and Kruskal Wallis and Mann Whitney - U statistical tests. Results: Statistical analysis revealed a significant difference between the toxicity of the test materials after 24-hr (P-0.025). The tests revealed that there was a significant difference between the cytotoxicity of MTA and Root - MTA (P=0.033), but there was no significant difference between MTA and PC (P=0.599) . Conclusion: MTA and PC show comparable biocompatibility when evaluated in vitro unlike Root - MTA which exhibits a higher toxicity. The results suggest that PC has potential for use as a less expensive root - end filling.