اندازه گيري فعاليت آنزيم 5-آمينولولينات دهيدراتاز اريتروسيت در بيماران با نارسايي مزمن كليوي

Assay of 5-aminolevalinate dehydratase in chronic renal failure patients

Background and Aim: 5- aminolevulinic acid dehydratase is the second enzyme in the heme biosynthetic pathway. This enzyme catalyzes the delta-aminolevulinic acid to prophobilinogen. Because there have been few studies in chronic renal failure, we thought it useful in patients with chronic renal failure. Thus, the goal of this study is to measure 5- aminolevalinate dehydratase activity in patients with chronic renal failure. Materials and Methods: Blood was obtained from patients with chronic renal failure for 5- aminolevulinate dehydratase activity determination in erythrocytes. Measurement was based upon the conversion of uminolevulinic acid to porphobilinogen, which is then measured spectrophotometrically. Results: Our results showed that 5-aminolevulinate dehydratase activity was (634.7±21.2 micromol/L/min) in patient group and in control group was (825.4±23.6 micromole/L/min). Conclusion: Our results showed that 5-aminolevulinate dehydratase activity was decreased in patients with chronic renal failure when compared to control group. These results showed that this enzyme may be inhibited in patients group.