اندازه گيري بنزن، تولوئن، اتيل بنزن و متا زايلن در ادرار به عنوان نمون هاي بيولوژيك به روش ميكرواستخراج مايع مايع پخشي و كروماتوگرافي گازي

Dispersive liquid-liquid microextraction combined with gas chromatography for determination of benzene, toluene, ethylbenzene and xylenes in urine as a biological sample

A method for the determination of benzene, toluene, ethylbenzene and xylenes urine of people not occupationally exposed to solvents is described. The dispersive liquid liquid microextraction technique combined with gas chromatography with a mass spectrometer detector is used. The sensitivity of recent mass spectrometers is good enough to furnish reliable results also in biological samples collected from the general population. No treatment for concentrating solvents present in urine is necessary. The main features of the method are easy preparation of biological samples, small volumes (7 ml), good repeatability and linearity in the range of interest. Detection limits were 0.004-0.01 |iig L"1 for benzene, toluene, ethylbenzene and w-xylene (BTEXs). The results obtained in biological samples from 25 woodworkers not occupationally exposed to BTEX (15 non-smokers and 10 smokers) are comparable to those obtained by other investigators.