وراثت پذيري مقاومت به زنبور ساقه خوار Cephus Cinctus Norton و شناسايي نشانگر ملكولي ميكروساتالايت پيوسته با آن در گندم دوروم

Heritability of Sawfly Resistance (Cephus Cinctus N.) and Microsatellite Markers Associated with it in Durum Wheat

Wheat stem sawfly (Cephus cinctus Norton) larvae reduces kernels per head, kernel weight and therefore grain yield by feeding within the stem and cutting vascular bundles, which are indispensable to nutrient flow. The present study was undertaken to estimate heratibility of sawfly resistance, evaluate the relationship between sawfly resistance and some agronomic as well as quality traits, and also to identify microsatellite markers linked with sawfly resistance in durum wheat (Triticum turgidum L. var durum). A set of 155 double haploid lines were taken record of percent stem sawfly cutting and some agronomic as well as quality traits in field trials within two years. Parents of double haploid population were tested with 517 wheat microsatellite markers. Primers that were polymorphic on the parents were tested on the whole population. Heritability estimate by variance of components indicated that genetics was the controling factor for the main part of stem sawfly cutting variations. Correlation coefficient of sawfly cutting with grain yield and 1000 grain weight was negative and highly significant within two years. Relationship of sawfly cutting and quality traits was not stable through the two years. Almost 10 percent of tested markers on the parents were polymorphic. Markers linkage map covered about 970 cM of durum genome. Results of t, F, %2 tests while using MQTL program showed a strong linkage between a markers group, including 3 markers, and sawfly cutting data. The linkage markers group is located on the long arm of chromosome 3B. There was no relationship observed between the markers linkage groups and other traits, then the markers can be used as marker-assisted selection to improve sawfly resistance without any negative effect on the other traits in durum wheat.