تاثير تنظيم كننده هاي رشد گياهي بر كالوس زايي و باززايي نيشكر واريته CP73-21

Effect of plant growth regulators on callus induction and regeneration of sugarcane variety CP73-21

In this study, in order to disinfect initial leaves of the sugarcane (Saccharum ojficinarum) variety CP73-2 I, five treatments were studied in a completely randomized design (CRO) with six replications. Sequential applications of 25% sodium hypochlorite for 10 minutes and 0.1% mercuric chloride for 30 seconds had the best results on disinfection of initial leaves. Six callus induction treatments were evaluated through a factorial experiment based on CRO with four replications. Murashig and skoog (MS) based medium supplemented with 3 mglʹ 2,4-0 along with 0.1 mglʹ and 0.3 mgl" BAP respectively, had the best results for callus induction of initial leaves. Regeneration of calli was studied using five treatments in a completely randomized design with four replications. Hormone- free modified MS (including casein hydrolyzate) and also modified MS with I rnglʹ BAP and 0.2 mgl" NAA were more efficient on regeneration. Root formation was studied using five treatments in a completely randomized design with four replications. Half- strength MS medium supplemented with om rnglʹ IBA was found as the best for root induction treatment. The regenerants passed different stages of acclimatization successfully.