اتصال آنتي بادي ضد مورفين به نانو ذره هاي طلاق تهيه شده با گلوتاميك اسيد

Conjugation of anti-morphine antibody to gold nanoparticles synthesized with glutamic acid

Background and Aim: Gold nanoparticles are one of the most applicable metal nanoparticles which are widely being used in different fields such as nanomedicine and nanobiotechnology. The goal of this study is the production ofgold nanoparticles with appropriate size by the use of Glutamic acid which is considered as a green method. For diagnostic application the synthesized gold nanoparticles would be than conjugated with anti-morphine antibody. Materials and Methods: In this experimental study, gold nanoparticles were synthesized and coated by chemical reduction method with the use of Glutamic acid. Formation of gold nanoparticles and their size were recognized and measured by UV-Vis spectroscopy, Dynamic light scattering and Transmission electron microscopy. Anti-morphine antibody was purified and prepared by subcutaneous injection of400 ug/ml dose of morphine vaccine to 9 rabbits. Study of immunity, immunogenicity and antibody production was performed by immuno-diffussion methods. After production and recognition of colloidal gold nanoparticles they were changed into suspension by the use of PBS buffer. They were then conjugated with anti-morphine antibody and incubated at 4ʹC for lIS hours. Conjugated nanoparticles were characterization by SDS-PAGE and UV-Vis spectroscopy. Results: The highest absorption of these particles was at 525 urn and their size was about 20 urn formed bands pertaining to conjugated nanoparticles were displaced in compare with standard samples. Conclusion: Glutamic acid is capable of synthesizing gold nanoparticles and since amino acids are intoxic, the nanoparticles which are synthesized by the use of amino acids, would be applicable in medicine and biotechnology. Nanoparticles conjugated with antibodies, could be also used as recognition tools.