تأثيراتانول برميزان رشد و تكوين جنين هاي دو سلولي متوقف شده موش

The effect of Ethanol on growth and development of two-cell arrested mouse embryo

Background: Arresting in certain step of developing like two cell block, could be the reason of infertility in some couples. Evaluate the effect of ethanol on growth and development of mouse two-cell arrested embryo is aimed of this study. Methods and Materials: In this experimental study 4-6 weeks old female mice were coupled with male mice following superovulation. Positive vaginal plaque mice were killed 48 hour after HCG injection. Two cell embryos were collected in RPMI medium and cultured in M16 medium and divided in three groups. The 2nd and 3rd groups were exposed to 4°C for 24 hour in order to delay and arrest for cleavage and developmental rate. The 2nd group (2nd control) were incubated immediately, while the 3rd group (experiment) were exposed to % 0.1 Ethanole for 5 minutes and the 1st group (1st control) without any exposure to low temperature were incubated. Results: The developmental rate of embryos exposed to low temperature (4°C) significantly decreased and induce, retardation and arrest (p=0.001). There were not significant difference between the groups mean of cleavage rate, but the mean percent of degenerated embryos between groups have significant differences (p=0.045). The mean percent of morulla were significantly different between groups (p=0.005). The mean percent of blastocyst and hatched blastocyst after 120 hr evaluation have significant differences between others groups (p=0.014) (p=0.001). Conclusion: Effect of % 0.1 ethyl-alchol on arrested two cell embryos can significantly increase the mean percent of morulla and development of blastocyst and hatching blastocyst stage in compare to control group, without any significant effect on cleavage rate